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您知道Chemgenes寡核苷酸時如何被合成的嗎？
發(fā)布時間： 2020-04-27 點擊次數(shù)： 1425次Chemgenes寡核苷酸是一類只有50個以下堿基的短鏈核苷酸的總稱（包括脫氧核糖核酸DNA或核糖核酸RNA內(nèi)的核苷酸），寡核苷酸可以很容易地和它們的互補鏈對接，所以常用來作為探針確定DNA或RNA的結(jié)構(gòu)，經(jīng)常用于基因芯片、電泳、熒光原位雜交等過程中。Chemgenes寡核苷酸合成的DNA（脫氧核糖核酸）可以用于鏈聚合反應(yīng)，能放大確定幾乎所有DNA的片段，在這個過程中寡核苷酸是作為引物，和DNA 中標記的互補片段結(jié)合，作成DNA的復(fù)制品。Chemgenes寡核苷酸如何被合成：寡核苷酸的制備是通過使用 DNA 合成儀完成的，而該儀器本質(zhì)上是一套由計算機控制的試劑遞送系統(tǒng)。第1個堿基將會被連接固體支持物（通常是玻璃或聚苯乙烯微珠），該支持物旨在錨定反應(yīng)柱中不斷延長的 DNA 鏈。DNA 合成由一系列的化學(xué)反應(yīng)所組成。Chemgenes寡核苷酸的儲存事項：凍干的寡核苷酸可在 –20°C 穩(wěn)定保存少 1 年。溶解于 TE 的寡核苷酸可在 –20°C 或 4°C 穩(wěn)定保存少 6 個月。在無核酸酶的情況下，溶解于水的寡核苷酸可在 –20°C 穩(wěn)定保存少 6 個月。確保所用的水具有中性 pH 以避免脫嘌呤作用。不要將溶于水的寡核苷酸在 4°C 下保存。
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