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北京華新康信與您分享Novus試劑盒操作進程的控制
發(fā)布時間： 2022-06-27 點擊次數(shù)： 820次Novus試劑盒是一種敏感性高，特異性強，重復性好的實驗診斷方法。由于其試劑穩(wěn)定、易保存，操作簡便，結(jié)果判斷較客觀等因素，已廣泛應(yīng)用在免疫學檢驗的各領(lǐng)域中。實驗前的準備工作：1、準備好所有實驗額外所需物品，如試管 吸頭 移液器 離心機等；2、確定試劑盒在有效期內(nèi)；3、仔細閱讀說明書；4、按說明書確定所有試劑齊全（數(shù)量 體積）；5、標本制備要規(guī)范，每份標本體積要按2-3個復孔以上的量制備（貯存），盡量分裝做備份；6、根據(jù)檢測標本數(shù)量確定所需試劑的量；7、按說明書將所用試劑盒平衡至室溫，配制所需試劑。Novus試劑盒的操作進程的控制：1、嚴格按照試劑說明書進行操作，操作前將試劑在室溫下平衡30～60min。2、加樣后及時放人孵箱，標本較多時，要分批操作，按說明進程嚴峻控制操作時間，避免孵育時間人為延伸，導致非特異性結(jié)合緊附于反應(yīng)孔周圍，難以清洗*。3、封板溫育時，各孔一定要封嚴，避免陽性標本的液體蒸發(fā)，發(fā)作周邊現(xiàn)象然后導致“花板”的出現(xiàn)。4、用洗板機洗板時，保證洗液注滿各孔，試劑盒洗板針疏通，洗完板后在吸水紙?zhí)暨x潔凈無或少塵的吸水材料上輕輕拍干：還要避免針口有纖維蛋白或異物，這些異物在洗板的進程中易發(fā)作拖帶現(xiàn)象而導致“花板”的出現(xiàn)。5、合理安排檢測量，避免反應(yīng)板過多構(gòu)成洗板等候時間長。6、加酶試劑后用吸水紙在酶標板外表輕拭吸干。7、顯色劑盡量在臨用前制作，堅持不用過期顯色劑，肉眼可見淺藍色的TMB顯色劑不用。8、加樣時保持顯色劑不外流；AB液應(yīng)避免觸摸金屬器械，加停止液時應(yīng)避免發(fā)作氣泡。9、Novus試劑盒應(yīng)保證清潔，整個操作進程中保證酶標板不觸摸次氯酸，以上的辦法可以使“花板”降至至低極限。然后提高檢測的特異性，并得到更可靠的實驗成果。
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