服務熱線
15201163601
-
Permagen試劑盒的檢測結果和判定標準
發布時間： 2022-11-28 點擊次數： 922次Permagen試劑盒的組合物由一個引物對和一個探針組成：引物對由序列表中序列1和序列2所示的兩條單鏈DNA分子組成：探針的序列為序列表中序列3。利用所提供的試劑盒對病毒進行檢測，具有操作簡便，快速、特異的優點。可快速篩查病毒，對口岸輸入性疾病篩查具有重要意義，為防御疾病的傳入做好技術支撐和戰略儲備，滿足當前衛生檢疫的需求。Permagen試劑盒檢測結果和判定標準：試驗結果成立的條件：陽性對照PCR反應產物電泳后在301bp的位置上出現特異性條帶；陰性對照PCR產物電泳后沒有條帶，試驗結果成立，否則結果不成立。結果判斷：在試驗結果成立的前提下，如果樣品PCR反應有特異性的條帶，則樣品為陽性；反之，為陰性。Permagen試劑盒性能檢測：1、Permagen試劑盒的重復性檢測對同一批提取的猴痘病毒陽性模板進行10次PCR擴增，觀察試劑盒對同一批次陽性模板擴增的結果。對10批提取的猴痘病毒陽性模板進行PCR擴增，觀察試劑盒對不同批次提取的陽性模板擴增的結果。2、Permagen試劑盒的穩定性檢測用配制好的檢測試劑盒分裝后保存在-20℃冰箱中，每隔7d對陽性模板進行PCR檢測，連續檢測6個月，觀察試劑盒在-20℃保存的穩定性。用配制好的PCR反應混合物分裝后分別保存在4℃冰箱或室溫中，每隔12h對陽性模板進行PCR檢測，連續檢測4d，觀察試劑盒在室溫或4℃保存的穩定性。用配制好的PCR反應混合物分裝后保存在-20℃冰箱后，分別凍融1~10次后，對陽性模板進行PCR檢測，觀察凍融次數對試劑盒的影響。
產品中心
Products
-
化學試劑
Origene parkell pan-biotech PAN lexogen Lampire labtidebiotech laborator labome ABI enzolifesciences emsdiasum EtonBioscience Equitech-bio kirgen invivogen integralmolecular Instech IndoorBiotechnologies INCELL IMMUSMOL G-Biosciences IMMUNOLOGICALS idtdna IMMUNODX ILLUMINA Immunodiagnostics IBALifeSciences Hypoxyprobe Horizon Cygnus Technologies Hellobio Greiner Cell Signaling Glycobiotech Corning FST Evrogen eurofins BioFire Defense biodatacorp atlasantibodies ATCC Astartebio ArcticZymes aobious anshlabs Advanced ChemTech Advaced Cell Diagnostics AdvanDx Agar Scientific ACBR TRc polyscience Fluka Gene Bridges fortebio Biocrick Fisher bioflux biomax beckman AmericanElements MOLTOX Permagen USA Scientific Chemgenes寡核苷酸 Asuragen vmrd抗體 Thermo asusragen AmplideX toxin polyplus signagen Berry&Associates ALZET滲透泵 alzet abcr abvector SGI-DNA Goldbio Iduron Sgidna Chromsystems Coriell GE Chemgenes Matreya Streck VMRD Sigma Cerilliant Innovative Cell Technologies Diagenode Trilinkbiotech Medkoo life diagnostics Gibco Neuromab全國總代 Novus BioLegend Bethyl Toxin Technology Listlabs Seracare 寶利嘉 賽默飛 安迪生物 行業專用試劑
-
生物試劑
Biosharp Sciex Seahorse Proteintech DiaMag complementtech PeproTech hooke Nzytech Pierce pepnet pelfreez PALL ozbiosciences OXOID ORFLO ommscientific omega nanopore ncbi NexcelomBioscience Mpbio MultiSciences MyBioSource Musechem MiltenyiBiotec minerva-biolabs microspheres-nanospheres microfil microbix modiquestresearch MOBITEC mercodia merckmillipore medicago JPT kerafast Kangchen Malvern magen Kapa Macrocyclics Macherey mabtech Lucigen leica Lifespan lifesensors lifeline life-ilab Lifecore life Licor advancedchemtech Affinity Abgent Abmgood ActiveMotif Acros Acrobiosystems enzymatics epicypher Elastin elabscience Echelon eBioscience eastcoast eaglebio E&K SCIENTIFIC ekfusa duran DSHB Dharmacon DGRC Dako Cypex comptech Encorbio Jetbiofil JCRBcellbank ISOSCIENCES INVIVOCHEM iris-biotech Full Moon BioSystems GBI FormnMax Finescience Favorgen CordenPharma CIL Chromadex Clodronateliposomes Chromogenix Chiron chemservice cellvis cellutron CELLTREAT cellscript cellbiolabs cellecta cedarlane cdnisotopes carbosynth cambio calbioreagents Buchem Boston Cellron Bioworld Biowest Biotium biostatus BiosPacific Biosearch BIOplastics Biooscientific BioPAL Biomol BIOMED Biomatik bioline Biocoat Bioclone Biocision biochain華新康信 biocell Biocare BIAseparations BDpharmingen Baseclick Badrilla B2S Life Sciences Axon Medchem AXIS-SHIELD avivasysbio avidity AVES-LABS Anatrace Americanbio AlphaDiagnoesticInternational Applied Genetics Laboratories Alstem Alomone AcceGen Akina AdvancedBioMatriX Adipogen Accuratechemical Academybiomed Abcam Zymo Research Addexbio ABL Abbexa Aablocks ZeptoMetrix Tocris TAKARA Spherotech Santa Roche Nist PBL MRC Holland rd-biotech Cellgs LC-LAB Greatcell Energy Hyclone Greerlabs GlycoSyn GlycoTech EY laboratories Cayman CanSyn Bioxcell Bio－Rad Agdia Abclonal amsbio auditmicro avantilipids Alsachim HTDialysis jetPRIME Carna Biosciences FItzgerald